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Literature summary extracted from

  • Fang, Q.; Kanugula, S.; Tubbs, J.L.; Tainer, J.A.; Pegg, A.E.
    Repair of O4-alkylthymine by O6-alkylguanine-DNA alkyltransferases (2009), J. Biol. Chem., 285, 8185-8195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expressed in Escherichia coli XL-1 Blue cells Escherichia coli
2.1.1.63 expressed in Escherichia coli XL-1 Blue cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.63 additional information construct human AGT-03 (where AGT sequence -V149CSSGAVGN157- is replaced with the corresponding Ogt -I143GRNGTMTG151-), exhibits enhanced DNA containing 4-O-methylthymine repair activity in vitro compared to wild type AGT Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Escherichia coli
-
-
-
2.1.1.63 Homo sapiens P16455
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 immobilized metal affinity column chromatography Escherichia coli
2.1.1.63 immobilized metal affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 4-O-methylthymine + [protein]-L-cysteine
-
 Escherichia coli DNA lacking 4-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Escherichia coli DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 additional information human AGT acts poorly on DNA containing 4-O-methylthymine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 AGT
-
 Homo sapiens
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Escherichia coli
2.1.1.63 O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens
2.1.1.63 OGT
-
 Escherichia coli